Was multiplied and the tk gene was cloned . the cloned tk gene was retrieved by proper restrictive hemodynamics . the retrieved tk gene was labeled by digoxin according to the kit of labeling and detection of digoxin . then , the specificity and sensitivity of tk gene probe were detected with dot blot hybridization . the sequence of tk gene of nm98a strain was analysed . the result of the analysis of tk gene ' s sequence confirmed that autoploidy between tk gene of nm - 98a strain and issued strain was 99 . 7 % 本研究中首次對iltv - nm98a株的tk基因進(jìn)行了克隆和序列分析,結(jié)果表明: iltv - nm98a株tk基因的核苷酸序列與已發(fā)表的iltvtk基因的核苷酸序列具有高度的同源性,兩者之間僅相差4個(gè)核苷酸,同源性高達(dá)99 . 7 ,從而證實(shí)了iltvtk基因是高度保守的,為iltvtk基因核酸探針的制備提供了有力的依據(jù)。